Saxion is part of the Dutch system of universities for higher vocational training and applied sciences. In this paper a case study is presented to gain more insight in spin offs of these types of universities, and their effect on the regional economy. For this purpose a database of 152 spin offs has been developed and a first survey was analysed. Most spin offs are active in the ICT and business services sectors. We have compared the results with the spin offs University of Twente. We observed that the percentage of spin offs, which was located outside the surrounding region of Saxion (33%) was significantly higher, than the percentage of spin off of the University of Twente (22%). The spin offs outside the region also have employees than Saxion spin off in the region. There is however no difference in the way the spin off perceive the business climate of their location. Stimulating entrepreneurship at universities of applied seems a good strategy to support the development of the promising knowledge intensive service sector in the eastern part of the |Netherlands. On the other hand this strategy should be accompanied with policy instruments aiming at convincing alumni entrepreneurs to stay in the region.